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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing

Fig. 3

Yeast growth in beechwood glucuronoxylan. (A) Growth of CRISPR/Cas9 engineered strains with BmXyn11A or XynB xylanases compared to XXX and XylA strains in Delft medium with 20 g L− 1 beechwood GX. (B) Growth of XynHB engineered strains compared to XXX and XylA strains in beechwood GX. (C) Growth of single strains compared to co-cultures (ration 1:1) with XylA strain in beechwood GX. (D) Growth of co-culture XylA + XylA-BmXyn11A strains (ratio 1:1) when supplemented with exogenous xylanolytic enzymes (200 µg/g GX). All strains were grown in triplicates. OD Equivalent = Optical density normalized from S. cerevisiae growth in Delft/glucose medium in a Growth-Profiler 960. GX = glucuronoxylan.

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Microbial Cell Factories

ISSN: 1475-2859

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