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Table 1 Plasmids constructed and used in this study

From: Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing

Plasmid Full Name

Description

Source

pJR1_01_SED1-XylA

TU1 that expresses Aspergillus oryzae SED1-XylA β-xylosidase under ScPGK1 promoter and ScTDH1 terminator with SED1 signal peptide (N-term)

This work

pJR1_02_SED1-BmXyn11A

TU1 that expresses Blastobotrys mokoenaii SED1-BmXyn11A xylanase under ScTDH3 promoter and ScPGK1 terminator with SED1 signal peptide (N-term)

This work

pJR1_03_SED1-XynHB

TU1 that expresses Bacillus sp. HBP8 SED1-XynHB xylanase under ScTDH3 promoter and ScPGK1 terminator with SED1 signal peptide

This work

pJR1_04_SED1-XynB

TU1 that expresses Aspergillus niger SED1-XynB xylanase under ScTDH3 promoter and ScPGK1 terminator with SED1 signal peptide

This work

pJR1_08_SED1-Agu115

TU1 that expresses Schizophyllum commune SED1-Agu115 α-glucuronidase under ScCCW12 promoter and ScADH1 terminator with SED1 signal peptide (N-term)

This work

pJR2_01_SED1-XylA-BmXyn11A

TU2 expressing genes from JR1_01 and JR1_02

This work

pJR2_02_SED1-XylA-XynHB

TU2 expressing genes from JR1_01 and JR1_03

This work

pJR2_03_SED1-XylA-XynB

TU2 expressing genes from JR1_01 and JR1_04

This work

pJR2_04_SED1-XylA-BmXyn11A-Agu115

TU3 expressing genes from JR1_01, JR1_02 and JR1_08

This work

pJR2_05_SED1-XylA-XynHB-Agu115

TU3 expressing genes from JR1_01, JR1_03 and JR1_08

This work

pJR2_06_SED1-XylA-XynB-Agu115

TU3 expressing genes from JR1_01, JR1_04 and JR1_08

This work