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Table 2 Strains constructed and used in this study

From: Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing

Strain

Abbreviated name

Description

Source

S. cerevisiae CEN.PK XXX

XXX

Expresses RPE1, TAL1, RKI1 and XKS1 genes, and insertion of codon optimized XYL1 and XYL2 genes from Scheffersomyces stipitis into the genome of parental strain S. cerevisiae CEN.PK 122 MDS

Westman et al. 2014

S. cerevisiae CEN.PK XXX-SED1.XylA

XylA

S. cerevisiae with secreted β-xylosidase activity

This work

S. cerevisiae CEN.PK XXX-SED1.BmXyn11A

BmXyn11A

S. cerevisiae with secreted xylanase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XynHB

XynHB

S. cerevisiae with secreted xylanase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XynB

XynB

S. cerevisiae with secreted xylanase activity

This work

S. cerevisiae CEN.PK XXX-SED1.Agu115

Agu115

S. cerevisiae with secreted α-methyl-glucuronidase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XylA-SED1.BmXyn11A

XylA-BmXyn11A

S. cerevisiae with secreted β-xylosidase and xylanase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XylA-SED1.XynHB

XylA-XynHB

S. cerevisiae with secreted β-xylosidase and xylanase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XylA-SED1.XynB

XylA-XynB

S. cerevisiae with secreted β-xylosidase and xylanase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XylA-SED1.BmXyn11A- SED1.Agu115

XylA-BmXyn11A-Agu115

S. cerevisiae with secreted β-xylosidase, xylanase and α-methyl-glucuronidase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XylA-SED1.XynHB-SED1.Agu115

XylA-XynHB-Agu115

S. cerevisiae with secreted β-xylosidase, xylanase and α-methyl-glucuronidase activity

This work

S. cerevisiae CEN.PK XXX-SED1.XylA-SED1.XynB- SED1.Agu115

XylA-XynB-Agu115

S. cerevisiae with secreted β-xylosidase, xylanase and α-methyl-glucuronidase activity

This work