Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Table 1 Overview of successful gene/BGC editing

	Strain	Locus tag	Size (kb)	Associated NP	Efficiency (%)
	Deletions
1	Photorhabdus laumondii TTO1	plu2234 (stlA)	1.9	Isopropylstilbene (1) [64]	100
3	Photorhabdus laumondii TTO1	plu3263 (gxpS)	15.4	GameXPeptides [46, 61]	100
4	Xenorhabdus nematophila ATCC 19061	XNC1_1711 (xcnA)	7.5	Xenocoumacins [62]	100
5	Xenorhabdus nematophila ATCC 19061	XNC1_2228–2230 (rdpA-C)	15.7	Rhabdopeptides [63]	100
	Promotor replacement/insertion
1	P. laumondii TTO1	ΔP_plu2186::P_vanCC	Δ0.2::1.3*	Indigoidine (2) [65]	100
2		ΔP_plu2234::P_vanCC	Δ3.4::1.3*	Isopropylstilbene (1) [64]	100
3		ΔP_plu3263::P_vanCC	Δ0.1::1.3*	GameXPeptide A (3) [46, 61]	74
4		ΔP_plu1881::P_vanCC	Δ0.08::1.3*	Glidobactins (4–5) [66]	68
5	X. nematophila ATCC 19061	ΔP_{XNC1_1711}::P_vanCC	Δ0.3::1.3*	Xenocoumacin I & II (7–8) [62]	100
6	X. nematophila ATCC 19061	ΔP_{XNC1_2228}::P_vanCC	Δ0.5::1.3*	Rhabdopeptides (9–12) [63]	100

* indicates the replaced sequence between homology arms by the 1.3 kb promoter sequence

ISSN: 1475-2859