Fig. 5
Identification of the Dieckmann cyclase gene bpdE by site-directed mutagenetic analysis. a) The documented proposal for the enzymatic mechanism of TE-like Dieckmann cyclases, in which the key active sites are labeled according to BpdE. b) The modeled protein structure of BpdE docked with a linear BPD precursor chain tethered in the Cys89 site of ligand binding pocket. c) HPLC analysis of compounds 1 and 2 from the RJ46 (ΔbpdE-C) strain complemented with the modified bpdE-C gene cassettes bearing sit-directed mutations in bpdE