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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Mutagenetic analysis of the biosynthetic pathway of tetramate bripiodionen bearing 3-(2H-pyran-2-ylidene)pyrrolidine-2,4-dione skeleton

Fig. 7

Improving BPD production by overexpressing the regulator gene bpd4. a) HPLC analysis of compound 1 from the RJ46 (ΔbpdE-C) strain complemented with a bpdE-C cassette derivative (Δbpd4_bpdE-C) with the regulator gene bpd4 deleted. b) HPLC analysis of compound 1 from the LHW50302 (W.T) introduced with extra copy of bpd4 gene engaged with a constitutive and strong promoter kasOp*. The production improvement levels of 1a and 1b were evaluated by calculating the target peak areas shown in the table at bottom

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Microbial Cell Factories

ISSN: 1475-2859

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