Fig. 2

Batch cultivation of T. reesei in a RWP at 1000 rpm. Scattered light and DO over time as well as pictures taken from the bottom and top of the cultivation plate at the end of cultivation for A wild type, B RutC30 and C RutC30 TR3158. Scattered light and DO showed high reproducibility between biological replicates for all strains. Compared to the previous cultivations, wall growth was drastically reduced and contact between cultivation suspension and sealing foil was successfully prevented. Cultivation conditions: RWP, Jourdier Medium, n = 1000 rpm, \({\text{d}_{0}}=3\) mm, 20 g l\(^{-1}\) glucose, \({\text{V}_{\text{W}}}=3.4\) ml, \({\text{V}_\text{L}}=1\) ml, humidity \(\ge 85\)%, \({\text{O}_{2}}=21\)%, T = 30 \(^\circ\)C, inoculum = 10\(^{5}\) spores .ml\(^{-1}\), \({\text{n}_\text{bio}}=6\)