Fig. 2

Microfluidics-based droplet generation and image processing for the evaluation of the fluorescence intensity of single yeast cells in microdroplets. The workflows pertain to the production of droplets encapsulating single yeast cells and to the microscopic image-processing method using machine learning. A The process of droplet generation using a flow-focusing microfluidic chip. B Image-processing method incorporating machine learning techniques for cell observation and evaluation of the fluorescence intensity