Fig. 4

Fluorescence-based detection of AGTR1 signaling stimulated by the secreted angiotensin analog peptides using engineered yeasts in droplet cultures. A Amino acid sequences of the Ang II peptide and its analogs. B GFP gray values of yeast cells contained in the P2′ region (Additional file 1: Fig. S4B) in droplet cultures at 9 h of incubation. The error bars represent the mean ± SD of more than 100 samples. Cultivation and analyses were performed using the same procedures as those described in Fig. 3. N.D. = not detected