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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Transcriptomics aids in uncovering the metabolic shifts and molecular machinery of Schizochytrium limacinum during biotransformation of hydrophobic substrates to docosahexaenoic acid

Fig. 8

Determination of SR21 cell’s oxidative state using stress biomarkers and non-enzymatic antioxidants after 72 h. (a) Hydrogen peroxide (H2O2) estimation as a reactive oxygen species (ROS) generated during β-oxidation, expressed in nmol g− 1 dried biomass; (b) Lipid peroxidation in terms of the malondiadehyde (MDA) content expressed in µmol g− 1 dried biomass; (c) Total phenolic (TPC) content of methanolic cell extracts expressed in mg gallic acid g− 1 dried biomass; (d) Total flavonoid content (TFC) of methanolic cell extracts expressed in mg quercetin g− 1 dried biomass; (e) Oxidized glutathione (GSSG) levels expressed in µmol g− 1 dried biomass; (f) Ratio of (GSH/GSSG) and (g) Relative expression of mitochondrial catalase (cat) and thioredoxin reductase (trx). The asterisks denote statistical significance * (p < = 0.05), ** (p < = 0.01), *** (p < = 0.001)

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Microbial Cell Factories

ISSN: 1475-2859

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