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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry

Fig. 3

Screening of GFP expression on plates. Top row, fluorescence imaging. Bottom row, white light imaging of the same plates. The left (plate 1) and middle (plate 2) column contain 48 clones each of Δ1-708 HaTRPA1, and the right (plate 3) column contains 48 clones of HaTRPA1. On each plate, the first clone is a low GFP control, and the second clone is a high GPF control from the first screen. Plate 1 clone 11, plate 2 clone 37 has similar intensity to the high control, and plate 3 clone 12 has higher intensity than the high control. Plate 1 clone 12 and 23, and plate 3 clone 28 and 35 have virtually no expression. On each plate clones are numbered starting from the top left

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Microbial Cell Factories

ISSN: 1475-2859

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