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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry

Fig. 4

Subcellular localization of GFP signal. Representative images of the GFP signal together with phase contrast signal (A, C), and GFP signal alone (B, D). In HaTRPA1 clones (A, B, exemplified by clone HaTRPA1 1.2.1) the fluorescence is localized more to the interior of the cell with some very bright speckles, whereas in Δ1-708 HaTRPA1 clones (C, D, exemplified by clone Δ1-708 HaTRPA1 2.2.37) it is localized around the edge of the cell. A single cell of each construct is highlighted and shown in a close up

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