Fig. 9

Fluorescent gels and Coomassie stained gels from the purification show what remains in each step of the purification. Both the samples, HaTRPA1 and Δ1-708 HaTRPA1, contain a lot of smaller bands throughout the purification. (A) HaTRPA1 does not show any bands that obviously correspond to the correct size of the construct, but after the IMAC there is a band marked by an arrowhead just below 100 kDa, which is far smaller than expected. (B) Δ1-708 HaTRPA1 in the meantime, contains a band around 60 kDa throughout the purification, which is somewhat smaller than expected. (1 and 8) PageRuler Plus Prestained Protein Ladder (Thermo Fisher). (2, 3, 9 and 10) Washed membrane, Coomassie stained and fluorescent gel. (4, 5, 11, and 12) Eluate from IMAC, Coomassie stained and fluorescent gel. (6, 7, 13 and 14) Fraction 29 from the SEC, Coomassie stained and fluorescent gel. The full gels are found in Figures S5-S10