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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc2 155 expressing mammalian steroidogenesis system

Fig. 2

Structures of the plasmids for gene expression in mycolicibacteria. a The plasmid pNS10 contains cDNA encoding bovine cytochrome P450scc. b The plasmid pNS11 contains cDNAs for bovine AdR, Adx (1–108 aa) and cytochrome P450scc in a single expression cassette driven by acetamidase promoter system (amiC, amiA, amiD and amiS). The plasmids include respective origins of replication (oriE and oriM) for propagation in E. coli and M. smegmatis, transcription terminator T1 from the E. coli rrnB gene (T), and hygromycin B resistance marker (hygR). NcoI, HindIII and BglII restriction sites used at cloning procedure are designated

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Microbial Cell Factories

ISSN: 1475-2859

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