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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc2 155 expressing mammalian steroidogenesis system

Fig. 6

The influence of adding acetamide on the bioconversion of MOM-cholesterol by M. smegmatis mc2 155 pNS11. a The yield of MOM-pregnenolone, b the yield of MOM-DHEA. Control—without adding acetamide; the single application of acetamide: at 0, 12 or 24 h; the double application of acetamide: at 0 and 12 h; 12 and 24 h or 0 and 24 h; the triple application of acetamide at 0, 12 and 24 h; the quadruple addition—at 0, 12, 24 and 36 h after the strain inoculation. The biotransformation time—48 h; final acetamide concentration in the medium—2 g/L

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Microbial Cell Factories

ISSN: 1475-2859

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