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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Multiplex genetic manipulations in Clostridium butyricum and Clostridium sporogenes to secrete recombinant antigen proteins for oral-spore vaccination

Fig. 1

C. sporogenes and C. butyricum secrete recombinant full-length NY-ESO-1 antigen. (A) The schema of the NY-ESO-1 expression plasmid, pPromoters-SpnprM3-NY-ESO-1, and the details of the used NY-ESO-1 expression clostridia. (−), Gram-negative replicons; (+), Gram-positive replicon; catP, thiamphenicol or chloramphenicol resistance gene; traJ, conjugal transfer gene; SP, signal peptide SpnprM3; M13-R/F, universal primer pair. Western blotting analysis of NY-ESO-1 expression in C. butyricum (B) and C. sporogenes (C). S, culture supernatant; L, pellet lysate; M, PageRuler Prestained Protein Ladder

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Microbial Cell Factories

ISSN: 1475-2859

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