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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Multiplex genetic manipulations in Clostridium butyricum and Clostridium sporogenes to secrete recombinant antigen proteins for oral-spore vaccination

Fig. 2

C. sporogenes and C. butyricum secrete CDP-NY-ESO-1, utilizing an AUU start codon. CDP is a reported chimeric designer peptide of E. coli origin. Using the primer pair M13-R/F, colony PCR was used to check the CDP-NY-ESO-1 expression plasmid with the AUG start codon (A) and the AUU start codon (B) in E. coli 10-β and S17-1. The Pfdx promoter was used, and the correct PCR size was 967 bp. The GeneRuler 1-kb DNA ladder was used as the molecular weight standard. (C) The used CDP-NY-ESO-1 expression clostridia and Western blotting analysis of CDP-NY-ESO-1 expression in C. butyricum and C. sporogenes. S, culture supernatant; L, pellet lysate; M, PageRuler Prestained Protein Ladder

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Microbial Cell Factories

ISSN: 1475-2859

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