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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Multiplex genetic manipulations in Clostridium butyricum and Clostridium sporogenes to secrete recombinant antigen proteins for oral-spore vaccination

Fig. 3

Genome-engineered clostridia secrete recombinant NY-ESO-1 and CDP-NY-ESO-1 antigens from the PyrE loci. The scheme of genome engineering in C. butyricum (A) and C. sporogenes-NT (B). Colony PCR to confirm the pyrE gene deletions and the integration of the NY-ESO-1 and CDP-NY-ESO-1 expression cassettes into the PyrE loci. The GeneRuler 1-kb DNA ladder was used as the molecular weight standard. (C) Western blotting analysis of the expression of recombinant NY-ESO-1 and CDP-NY-ESO-1 in genome-engineered clostridia. S, culture supernatant; L, pellet lysate; M, PageRuler Prestained Protein Ladder

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Microbial Cell Factories

ISSN: 1475-2859

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