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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Multiplex genetic manipulations in Clostridium butyricum and Clostridium sporogenes to secrete recombinant antigen proteins for oral-spore vaccination

Fig. 5

Genetically manipulated clostridia secrete recombinant SARS-CoV-2-related antigens. The schema and strain lists for the secretion of full-length Spike_S1 (A) and bacterial adjuvant (CDP)-fused Spike_S1 (D) antigens in clostridia. Western blotting analysis of the expression of recombinant full-length Spike_S1 in C. butyricum (B) and C. sporogenes (C) and the expression of recombinant CDP-Spike_S1 in C. butyricum (E) and C. sporogenes-NT (F). P, purified SARS-CoV-2 S1 protein produced in HEK293 cells; S, culture supernatant; L, pellet lysate; M, PageRuler Prestained Protein Ladder

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Microbial Cell Factories

ISSN: 1475-2859

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