Fig. 3

Effect of gene ptsG deletion on production of 1,2,4-BT from xylose. A Time-course of fed-batch fermentation in the 300 mL shake flask by E. coli BT-5 at 180 rpm and 37 °C. B Time-course of fed-batch fermentation in the 300 mL shake flask by E. coli BT-6 at 180 rpm and 37 °C. C Time-course of fed-batch fermentation in 300 mL shake flask by E. coli BT-6 at 180 rpm and 30 °C. Xylose at the concentration of 10 g/L was added in the medium when necessary. Values are the average ± SD (n = 3 independent experiments)