Fig. 5

Effect of pgi deletion on 1,2,4-BT production from xylose. A Time-course of fed-batch fermentation by E. coli BT-9 in 1-L bioreactor. B Time-course of fed-batch fermentation by E. coli BT-10 in 1-L bioreactor. The fed-batch fermentation was conducted in 1-L bioreactor containing 0.8 L LB broth with 30 g/L xylose, 10 g/L glucose and 10 g/L lactose at 400 rpm, 1.5 vvm and 30 °C. Xylose concentration was adjusted to 30 g/L when lower than 10 g/L. The experiments were conducted in triplicate. Two representative time-courses of E. coli BT-9 A and E. coli BT-10 (B) are reported herein