Fig. 6

1,2,4-BT production through fed-batch fermentation with E. coli BT-10 using xylose or CCH as the substrate. A Time-course of fed-batch fermentation by E. coli BT-10 in 7.5-L bioreactor with xylose as the substrate. The experiment was conducted in 7.5-L bioreactor with 5 L LB broth containing 30 g/L xylose, 10 g/L glucose and 10 g/L lactose at 400 rpm, 1.5 vvm and 30 °C. Xylose concentration was adjusted to 30 g/L when lower than 10 g/L. The experiment was conducted in triplicate. A representative time-course is reported herein. B Time-course of fed-batch fermentation by E. coli BT-10 in 7.5-L bioreactor with CCH as the substrate. The experiment was conducted in 7.5-L bioreactor containing 5 L LB broth with CCH (the final concentration of xylose was adjusted to 30 g/L) and whey powder (the final concentration of lactose was adjusted to 10 g/L) at 400 rpm, 1.5 vvm and 30 °C. Xylose concentration was adjusted to 30 g/L when lower than 10 g/L. The experiment was conducted in triplicate. A representative time-course is reported herein