Table 1 Bacterial strains and plasmids used in this work

Strain

E. coli DH5α

F^– φ80lacZ∆M15 ∆(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(r_K^–, m_K⁺) phoA supE44 λ^– thi-1 gyrA96 relA1

Novagen

E. coli 0K

E. coli W3110 (DE3)

[27]

E. coli BT-1

E. coli 0K containing plasmid pETP_tac-xylBC

This study

E. coli 1K

E. coli W3110 (DE3)ΔxylA

[27]

E. coli BT-2

E. coli 1K containing plasmid pETP_tac-xylBC

This study

E. coli 3K

E. coli 1KΔyjhHΔyagE

[27]

E. coli BT-3

E. coli 3K containing plasmid pETP_tac-xylBC

This study

E. coli 4K

E. coli 3KΔxynR

[27]

E. coli BT-4

E. coli 4K containing plasmid pETP_tac-xylBC

This study

E. coli 4KI

E. coli W3110 (DE3)ΔxylAΔyjhHΔyagEΔxynR with P_T7-xylD-kdcA expression cassette knocking in the position of xynR in chromosome

[27]

E. coli BT-5

E. coli 4KI containing plasmid pETP_tac-xylBC

This study

E. coli 5KI

E. coli 4KIΔptsG

This study

E. coli BT-6

E. coli 5KI containing plasmid pETP_tac-xylBC

This study

E. coli BT-7

E. coli 5KI with P_T7-xylBC knocking in the position of ptsG in chromosome

This study

E. coli BT-8

E. coli 5KI with P_T7-xylBC knocking in the position of mgsA in chromosome

This study

E. coli BT-9

E. coli 5KI with xylBC knocking in the position of xylA in chromosome

This study

E. coli BT-10

E. coli BT-9Δpgi

This study

Plasmid

pETP_tac-xylBC

Expression vector, pETP_tac with gene xylBC

[7]

pTKRED

Plasmid expressing Red recombinase genes; Spe^r

Addgene

pCP20

Plasmid expressing Flp recombinase to remove kan cassette; Cm^r

CGSC^b

pKD4

Template for kanamycin resistance cassette; Kan^r

CGSC^b